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    • 专利摘要:
    Formulation, or tissue adhesive, obtained from a blood composition rich in platelets and/or growth factors, and method of preparing said adhesive. The method of preparing the adhesive comprises the steps of raising the temperature of the initial blood composition and subsequently activating the composition. Among other advantages, the tissue adhesive is biocompatible and biodegradable, it has desirable biological or medical properties provided by the presence of platelets or growth factors, and it also has high adhesiveness and an accelerated coagulation process. (Machine-translation by Google Translate, not legally binding)
    公开号:ES2778798A1
    申请号:ES201930106
    申请日:2019-02-11
    公开日:2020-08-11
    发明作者:Aldecoa Eduardo Anitua
    申请人:BTI Biotechnology Insttitute；
    IPC主号:
    专利说明:

    [0002] FORMULATION OR TISSUE ADHESIVE OBTAINED FROM A BLOOD COMPOSITION CONTAINING PLATELETS, AND METHOD OF PREPARING SUCH FORMULATION
    [0004] Technical sector
    [0006] The invention relates to a formulation with desirable biological or medical properties, obtained from an initial blood composition containing platelets. The invention also relates to a method of preparing said formulation. The formulation serves as a tissue adhesive.
    [0008] State of the art
    [0010] In the state of the art, it is known to prepare compositions from human or animal blood, where the blood is processed in such a way as to obtain a plasma rich in platelets (PRP) and / or a plasma rich in growth factors that they have useful biological and medical properties. Said PRP or plasma rich in growth factors have been used successfully in ex vivo applications, for example as a cell culture medium, and in vivo, for example to carry out a bone regeneration process in a patient or to treat a patient with a joint ailment through infiltrations. In the case of compositions intended for in vivo applications, the technology for preparing PRP formulations and plasma rich in growth factors has evolved towards the preparation of autologous compositions, that is, obtained from the patient's own blood. Examples of this type of compositions and preparation methods can be found in patents US6569204 and ES2221770.
    [0012] Compositions consisting of platelet-rich fibrin (PRF), obtained from blood, are also known. Like the plasmas cited above, fibrin can be autologous or heterologous. Unlike plasma, which is liquid, fibrin has a solid or semi-solid consistency.
    [0014] An example of fibrin is known as fibrin gel or fibrin mesh, which is a formulation whose semi-solid consistency is very convenient for certain applications. The fibrin gel or mesh preparation procedure generally begins with a first phase in which a PRP or plasma rich in growth factors is obtained by an applicable method, for example by centrifuging blood drawn from a patient until the blood separates in several fractions, and extracting the upper fraction, that is the fraction of platelet rich plasma (PRP) or plasma rich in growth factors. Subsequently, the platelets contained in the PRP or plasma rich in growth factors are activated (activation being understood as the action of causing the platelets to release certain growth factors contained within), for example by adding calcium chloride. As a consequence of the activation, and if a sufficient time is waited, the eventual polymerization of fibrin occurs from the fibrinogen contained in the plasma, obtaining a final compound that is a fibrin clot (also called fibrin gel or mesh due to its semi-solid consistency, like a kind of biological sponge). This procedure is usually performed to obtain a fibrin gel from blood modified with an anticoagulant, such as sodium citrate. But blood can also be processed without mixing it previously with anticoagulant; in this case, by centrifuging the blood, it is possible to separate the plasma from the red blood cells and, at the same time, to obtain the fibrin gel without the need to add calcium chloride or another platelet activator. Some examples of fibrin gel or mesh application are: forming a biological scaffold to fill bone defects; be applied on wounds or lesions for the progressive release of growth factors; be used as a matrix for stem cell culture; be used as a membrane to close defects or ulcers; be used in the manufacture of tissues, which is known as tissue engineering, where, in addition to cells and growth factors, it is especially important to have a matrix or scaffold where cells can grow.
    [0015] However, platelet-rich preparations (PRP, plasma rich in growth factors, PRF) have limited capacity as a tissue adhesive. The importance of having an adequate adhesive property is highlighted in that surgical and chronic wounds represent a global socio-economic burden, often underestimated, both for patients and for health systems. One of the main concerns that we find at this level are the profuse and continuous bleeding that may occur during surgery or in a chronic wound, and the postoperative discomfort and complications derived from surgical sutures, such as suture abscesses, formation of granulomas or tissue necrosis. Over the years, a wide range of treatment modalities have emerged in the world of surgery with the aim of reducing these complications, among these treatments is the use of fibrin glue / sealant.
    [0017] Commercial allogeneic fibrin sealants represent a highly suitable non-invasive alternative. However, although they work efficiently, their cost is high and they may not be available in every country or region. In addition, because the commercial allogeneic fibrin sealant is obtained from human plasma, there is a risk of transmission of certain diseases and hypersensitivity reactions.
    [0019] The safest way to prepare fibrin sealant is to obtain it from the patient's own blood. But, the preparation time (usually using freezing or lyophilization techniques) is long, requiring at least 24 hours for processing, so it cannot be done during surgery or requires that the patient come the day before surgery. to draw blood from you. These freezing or lyophilization techniques are based on achieving an increase in the fibrinogen concentration and suffer from limitations such as a low concentration of fibrinogen and coagulation proteins, so the sealing time is long and highly variable due to variability biological that exists in each patient. Other methodologies used to expedite the preparation of a autologous fibrin sealant use chemicals to promote the precipitation of fibrinogen, but these products can produce irritative and inflammatory processes in the application tissues.
    [0021] The present invention aims to achieve a formulation with desirable biological or medical properties, obtained from an initial blood composition rich in platelets and / or growth factors, which can be prepared in surgical times and an increased tissue adhesiveness. Among other applications, the formulation is intended to serve as an alternative to commercial fibrin sealant.
    [0023] Brief description of the invention
    [0025] The object of the invention is a formulation with desirable biological or medical properties, which comprises or is derived from an initial blood composition (of human or animal origin; autologous, homologous or heterologous), rich in platelets and / or growth factors and which comprises proteins from the initial blood composition itself, with the particularity that the formulation presents an increased adhesiveness. This formulation can be autologous (it is prepared and applied to the same donor), homologous (the donor and the recipient are of the same species) or heterologous (the donor and recipient are of different species and could be qualified as a "sealant of fibrin ”(using a terminology analogous to that used to refer to the application of fibrin preparations for sealing) due to its increased adhesiveness and accelerated coagulation. The composition presents a new morphological and biomechanical configuration compared to the rest of sealants of fibrin, blood compositions rich in platelets and / or growth factors, and the like known in the state of the art.
    [0027] The formulation according to the invention is biocompatible, biodegradable and exhibits the desirable biological or medical properties provided by the presence of platelets or growth factors. In addition, the formulation exhibits increased tissue adhesiveness and is obtained in short times. The formulation according to the invention is therefore an advantageous alternative to conventional fibrin sealant, due to the fact that the formulation is autologous, adhesive and is obtained in a short time without the addition of chemicals. Furthermore, the formulation exhibits good compressive adhesiveness, similar to or better than the conventional allogeneic fibrin sealant Tisseel® and PRP (commonly used as a sealant), and adequately supports the resistance that a tissue can exert; therefore, the formulation is very suitable for use as a fibrin sealant. In addition, it is injectable.
    [0029] Another object of the invention is a method of preparing the above formulation, wherein said method comprises the steps of: having an initial blood composition rich in platelets and / or growth factors whose base formulation can vary; heating the blood composition to a temperature of 40 to 55 ° C; centrifuge the initial blood composition for at least 1 minute; reduce the volume of the initial composition. This method of the invention also comprises the addition of a platelet activating substance and the formation of fibrin to obtain a blood composition rich in platelets and / or growth factors in gel form.
    [0031] Brief description of the figures
    [0033] The details of the invention can be seen in the accompanying figures, these are not intended to be limiting of the scope of the invention:
    [0035] - Figure 1 shows the coagulation time of different examples of formulations according to the invention.
    [0036] - Figure 2 shows the adhesiveness of different examples of formulations according to the invention.
    [0037] - Figure 3 shows the effect of the activator and platelets on the clotting time of the formulations according to the invention. - Figure 4 shows the effect of the activator and platelets on the adhesiveness of different examples of formulations according to the invention.
    [0038] - Figure 5 shows the effect of the activator and the efficacy of different examples of formulations according to the invention and in comparison with the commercial sealant Tisseel® in the adhesiveness.
    [0039] - Figure 6 shows the efficacy as a tissue adhesive of different examples of formulations according to the invention compared to the commercial sealant Tisseel® as a tissue adhesive.
    [0041] Detailed description of the invention
    [0043] In order to overcome problems still existing in the state of the art related to the adhesiveness of PRPs, an alternative formulation with desirable biological or medical properties and with improved adhesiveness is proposed. Said formulation comprises or is derived from an initial blood composition containing platelets. This composition is adhesive due to heat treatment and formation of a fibrin clot. The sealant prepared according to this invention has been found to have a tissue adhesiveness similar to the Tisseel® fibrin sealant and better than the adhesiveness of a conventional platelet-rich fibrin or platelet-rich plasma.
    [0045] The initial blood composition can be, for example, a platelet-rich blood plasma, that is, a plasma with a high concentration of platelets. Said plasma has generally been obtained through the technique of centrifuging blood (to separate it into a fraction of a fraction of red blood cells, a fraction of white blood cells and a fraction of platelet-rich plasma (PRP)) and separating all or part of the fraction of platelet rich plasma (PRP).
    [0047] The initial blood composition may or may not contain leukocytes.
    [0049] For the activation of the initial blood composition one or more of: calcium chloride, thrombin, sodium gluconate, collagen, supernatant (a liquid substance that appears on the clot when causing coagulation of a platelet rich plasma (PRP) ) and its subsequent retraction), supernatant of a blood plasma rich in growth factors, or any other agent that acts by activating platelets and inducing fibrin formation in such a way that the platelets have released certain growth factors from within.
    [0051] A method of preparing a formulation with desirable biological or medical properties is also proposed, wherein said method comprises the steps of:
    [0053] a) have an initial blood composition rich in platelets and / or growth factors with or without anticoagulant, which is preferably a plasma rich in platelets with or without leukocytes, or a plasma rich in growth factors with or without leukocytes.
    [0055] b) Raise the temperature of the initial composition to a temperature of 40 to 55 ° C.
    [0057] c) Centrifuge the initial blood composition for at least 1 minute.
    [0059] d) Remove at least part of the plasma fraction obtained as a result of centrifugation.
    [0061] e) Activate the blood composition that remains after removing at least part of the plasma fraction as indicated in step d). Activation can be performed, for example, by adding calcium chloride, thrombin, a combination of calcium chloride and thrombin, sodium gluconate, collagen, supernatant (a substantial liquid that appears on the clot when coagulation of a rich plasma is caused platelets (PRP) and its subsequent retraction), supernatant of a blood plasma rich in growth factors and / or any other platelet activating agent. As a consequence, platelets are activated and fibrin formation is induced so that platelets release from their interior certain growth factors.
    [0063] The above method produces a precipitation of protein substances without the denaturation of fibrinogen as evidenced by the obtaining of a fibrin clot after activation. By removing part of the volume from the initial composition, the concentration of these protein substances is increased. In fact, and in addition, the method produces a remarkable acceleration in the coagulation of the blood composition and in its adhesion strength. In summary, as a consequence of the thermal process, new biocompatible and biodegradable formulations are achieved, with two main advantages: a short coagulation time and a higher adhesiveness, which makes this formulation can be applied as an adhesive or fibrin sealant.
    [0065] Preferably, the temperature of the initial blood composition is increased to a temperature in the range of 40 to 53 ° C.
    [0067] The initial blood composition rich in platelets and / or growth factors can be of human or animal origin. In addition, it can be autologous (belonging to a patient whom it is desired to treat later with the final formulation), homologous (belonging to a member of the same species as the patient, the patients, the cells or another biological entity to be treated. or processed with the final formulation) or heterologous (belonging to a member of a different species than the patient, patients, cells or other biological entity that is to be treated or processed with the final formulation).
    [0069] The invention contemplates that the initial blood composition may optionally incorporate one or more additional substances, added prior to the claimed heat treatment. These additional substances can be:
    [0071] - one or more bioactive agents selected from proteins, peptides, nucleic acids, polysaccharides, lipids, non-protein organic substances and inorganic substances;
    [0072] - one or more biodegradable polymers selected from: acid hyaluronic, hyaluronate salts, chondroitin 4 sulfate, chondroitin 6 sulfate, dextran, silica gel, alginate, hydroxypropyl methylcellulose, chitin derivatives, preferably chitosan, xanthan gum, agarose; polyethylene glycol (PEG), polyhydroxyethylene methacrylate (HEMA), synthetic or natural proteins, collagens;
    [0073] - one or more organic polymers selected from the group of polycarpolactone, polyglycolic, polylactic, and their co-polymers; - one or more of the following agents: antibiotics, antimicrobials, anticancer agents, analgesics, growth factors, hormones;
    [0074] - one or more inorganic components selected from the group of calcium salts, magnesium salts, and / or strontium salts.
    [0076] The invention also contemplates that any of the above substances can be added to the formulation after the heat treatment has been carried out.
    [0078] The formulation according to the invention contemplates various embodiments in which the formulation may comprise, in addition to the technical aspects claimed, other compounds, components, molecules, etc. that are convenient for the specific application for which the formulation is intended.
    [0080] Furthermore, it is possible to perform additional steps on the formulation produced according to the method described in this invention that include desiccates to increase its versatility. That is, prior to its activation (activation of platelets and formation of fibrin), the formulation according to the invention can be dried (dry heat) or lyophilized. This formulation can be subsequently rehydrated by different alternatives such as adding a saline solution, a plasma rich in platelets, a supernatant of a plasma rich in platelets, a plasma rich in growth factors, a supernatant of a plasma rich in growth factors, or any other liquid substance.
    [0081] Examples
    [0083] Example 1
    [0085] The starting point is a sample of 9 tubes (9ml) that hermetically contain blood drawn from a patient. The tubes are centrifuged at a speed of 580 g, for a time of 8 minutes and at room temperature. As a consequence of centrifugation, the blood contained in each tube is divided into several fractions. The upper fraction, or platelet-rich plasma (PRP) fraction, is extracted into a white tube, obtaining a total of 36 ml of plasma. The plasma is divided into 6 tubes, each tube containing 6 ml of plasma. Next, the temperature of each of the 6 tubes is raised to 37.55, 45.95, 51.05, 52.4, 53.9, and 55.35 ° C, respectively. Subsequently, the 6 tubes are centrifuged at a speed of 580 g, for a time of 8 minutes and at room temperature, producing the precipitation of platelets and new protein substances. To concentrate these protein substances and after centrifugation of the heated plasma, the upper half of the plasma is removed. Finally, the precipitate is resuspended in the plasma that has remained in the tube.
    [0087] Next, the formulations in the 6 tubes are activated by adding a PRP supernatant (333pl) and 20 µl of calcium for each 1ml of formulation, which initiates the formation of fibrin in the formulations.
    [0089] Clotting time (the time it takes for the blood composition to change its state from liquid to gel) due to fibrin formation has been measured. Figure 1 shows the ability of the method of the invention to accelerate clotting time. It should be noted that the clotting time of a conventional PRP, activated in the same way as the previous formulations according to the invention (that is, with PRP supernatant (333pl) and 20 µl of calcium per 1ml) has been 4 ,5 minutes. As can be seen in the graph, the formulations according to the invention have lower or accelerated coagulation times with respect to said conventional PRP. Said acceleration of coagulation is greatest for the temperature of 51.05 ° C, followed by the temperatures of 52.4 and 53.9 ° C. A stable coagulum has not been obtained when the temperature is 55.3 ° C.
    [0091] Example 2
    [0093] The starting point is a sample of 9 tubes (9ml) that hermetically contain blood drawn from a patient. The blood is centrifuged at a speed of 580 g, for a time of 8 minutes and at room temperature. As a consequence of centrifugation, the blood contained in each tube is divided into several fractions. The upper fraction, or platelet-rich plasma (PRP) fraction, is extracted into a white tube, obtaining a total of 36 ml of plasma. The plasma is divided into 6 tubes, each tube containing 6 ml of plasma. Next, the temperature of each of the tubes is raised to 37.55, 45.95, 51.05, 52.4, 53.9 and 55.35 ° C, respectively. Subsequently, it is centrifuged at a speed of 580 g, for a time of 8 minutes and at room temperature, producing the precipitation of platelets and new protein substances. To concentrate these protein substances and after centrifugation of the heated plasma, the upper half of the plasma has been removed. Finally, the precipitate is resuspended in the plasma that has remained in the tube.
    [0095] Next, the formulations in the 6 tubes are activated by adding a PRP supernatant (333pl) and 20 µl of calcium for each 1ml of formulation, which initiates the formation of fibrin in the formulations.
    [0097] Two glass slides have been glued with the formulation after it was activated. After coagulation, the glued slides were incubated in distilled water for 3 minutes and then the adhesion strength of the formulation was measured using gram weights. Figure 2 shows the adhesion strength of the formulations. The highest adhesion force is that corresponding to the temperature of 51.05 ° C, followed by the temperatures of 37.55 and 45.95 ° C. The lowest adhesion force is for the temperature of 55.3 ° C, followed by that of 53.9 ° C.
    [0098] Example 3
    [0100] The starting point is a sample of 9 tubes (9ml) that hermetically contain blood drawn from a patient. The tubes are centrifuged at a speed of 580 g, for a time of 8 minutes and at room temperature. As a consequence of centrifugation, the blood contained in each tube is divided into several fractions. The upper fraction, or platelet-rich plasma (PRP) fraction, is extracted into a white tube, obtaining a total of 36 ml of plasma. The plasma is divided into 6 tubes, each tube containing 6 ml of plasma. Samples are processed according to the following:
    [0102] - Control sample: PRP that is activated with calcium ions in a ratio of 20 µl of 10% calcium chloride for each 1ml of PRP.
    [0103] - Control-activator sample: PRP that is activated with PRP supernatant (333 µl) and 20 µl of 10% calcium chloride for each 1 ml of PRP.
    [0104] - Sample control-method: PRP that is centrifuged at a speed of 580 g, for a time of 8 minutes and at room temperature. 2/3 of the initial volume is removed and the platelet precipitate is resuspended in the remaining 1/3 of the initial volume. It is activated with PRP supernatant (333 µl) and 20 µl of 10% calcium chloride for each 1 ml of PRP.
    [0105] - Sample formulation 1: The temperature of PRP is raised to 51 ° C.
    [0106] Subsequently, it is centrifuged at a speed of 580 g, for a time of 8 minutes and at room temperature. 2/3 of the initial volume is removed and the platelet and protein precipitate is resuspended in the remaining 1/3 of the initial volume. The formulation is activated with the PRP supernatant (333 µl) and 20 µl of 10% calcium chloride for each 1 ml of PRP.
    [0107] - Sample formulation 2: The temperature of PRP is raised to 51 ° C.
    [0108] Subsequently, it is centrifuged at a speed of 580 g, for a time of 8 minutes and at room temperature. 1/2 of the initial volume is removed and the precipitate is resuspended platelet and protein in the remaining 1/2 of the initial volume. The formulation is activated with the PRP supernatant (333 µl) and 20 µl of 10% calcium chloride for each 1 ml of PRP.
    [0109] - Sample formulation 3: The temperature of PRP is raised to 51 ° C.
    [0110] Subsequently, it is centrifuged at a speed of 580 g, for a time of 8 minutes and at room temperature. The platelet and protein precipitate is resuspended in the total initial volume. The formulation is activated with the PRP supernatant (333 µl) and 20 µl of 10% calcium chloride for each 1 ml of PRP.
    [0111] - Formulation sample 4: Platelets are removed from PRP by filtration with filters with pore size of 20 µl. Then the temperature of the PRP is raised to 51 ° C. Subsequently, it is centrifuged at a speed of 580 g, for a time of 8 minutes and at room temperature. 2/3 of the initial volume is removed and the platelet and protein precipitate is resuspended in the remaining 1/3 of the initial volume. The formulation is activated with the PRP supernatant (333 µl) and 20 µl of 10% calcium chloride for each 1 ml of PRP.
    [0113] The results of the clotting time in Figure 3 indicate that: the use of the activator thrombin (PRP supernatant) calcium, used in the control-activator, control-method and formulations 1-4, accelerates the clotting of the PRP with respect to the use calcium ion only (control sample). Also a second centrifugation of the PRP before activation (control-method and formulations 1-4) further accelerates the coagulation possibly due to the increase in the concentration of platelets by removing part of the initial volume. However, the method according to the invention accelerates coagulation independently of the platelet concentration as shown by the results of formulation 3 (without increase in platelet concentration) and formulation 4 (without platelets). The shorter clotting times were those corresponding to formulations 1 and 2. Thus, the clotting time indicates the novelty and effectiveness of the method of the invention to accelerate the clotting process.
    [0114] Example 4
    [0116] The starting point is a sample of 9 tubes (9ml) that hermetically contain blood drawn from a patient. The tubes are centrifuged at a speed of 580 g, for a time of 8 minutes and at room temperature. As a consequence of centrifugation, the blood contained in each tube is divided into several fractions. The upper fraction, or platelet-rich plasma (PRP) fraction, is extracted into a white tube, obtaining a total of 36 ml of plasma. The plasma is divided into 6 tubes, each tube containing 6 ml of plasma. Samples are processed according to the following:
    [0118] - Control sample: PRP that is activated with calcium ions in a ratio of 20 µl of 10% calcium chloride for each 1 ml of PRP. - Control-activator sample: PRP that is activated with PRP supernatant (333 µl) and 20 µl of 10% calcium chloride for each 1 ml of PRP.
    [0119] - Sample control-method: PRP that is centrifuged at a speed of 580 g, for a time of 8 minutes and at room temperature. 2/3 of the initial volume is removed and the platelet precipitate is resuspended in the remaining 1/3 of the initial volume. It is activated with PRP supernatant (333 µl) and 20 µl of 10% calcium chloride for each 1 ml of PRP.
    [0120] - Sample formulation 1: The temperature of PRP is raised to 51 ° C.
    [0121] Subsequently, it is centrifuged at a speed of 580 g, for a time of 8 minutes and at room temperature. 2/3 of the initial volume is removed and the platelet and protein precipitate is resuspended in the remaining 1/3 of the initial volume. The formulation is activated with the PRP supernatant (333 µl) and 20 µl of 10% calcium chloride for each 1 ml of PRP.
    [0122] - Sample formulation 2: The temperature of PRP is raised to 51 ° C.
    [0123] Subsequently, it is centrifuged at a speed of 580 g, for a time of 8 minutes and at room temperature. 1/2 of the initial volume is removed and the platelet and protein precipitate is resuspended in the remaining 1/2 of the initial volume. I know activates the formulation with PRP supernatant (333 gl) and 20 gl of 10% calcium chloride for each 1 ml of PRP.
    [0124] - Sample formulation 3: The temperature of PRP is raised to 51 ° C.
    [0125] Subsequently, it is centrifuged at a speed of 580 g, for a time of 8 minutes and at room temperature. The platelet and protein precipitate is resuspended in the total initial volume. The formulation is activated with the PRP supernatant (333 gl) and 20 gl of 10% calcium chloride for each 1 ml of the PRP.
    [0126] - Formulation sample 4: Platelets are removed from the PRP by filtration with filters with pore size of 20 gl. Then the temperature of the PRP is raised to 51 ° C. Subsequently, it is centrifuged at a speed of 580 g, for a time of 8 minutes and at room temperature. 2/3 of the initial volume is removed and the platelet and protein precipitate is resuspended in the remaining 1/3 of the initial volume. The formulation is activated with the PRP supernatant (333 gl) and 20 gl of 10% calcium chloride for each 1 ml of the PRP.
    [0128] Two glass slides have been glued with the samples described above after their activation. The samples have been incubated in distilled water and then the strength of the adhesion of the formulation has been measured using weights in grams. Figure 4 shows the adhesion strength of the formulations. The results clearly indicate that the improvement in adhesion occurs only in the formulations according to the present invention (formulations 1 and 2) since the use of calcium thrombin (control-activator) or increasing the platelet concentration (control-method) have not improved adhesion of PRP activated with calcium ions. The best adhesion has been obtained by formulations 1 and 2 of the present invention.
    [0130] Example 5
    [0132] The starting point is a sample of 8 tubes (9ml) that hermetically contain blood drawn from a patient. The tubes are centrifuged at a speed of 580 g, for a time of 8 minutes and room temperature. As a consequence of centrifugation, the blood contained in each tube is divided into several fractions. The upper fraction, or platelet-rich plasma (PRP) fraction, is extracted into a white tube, obtaining a total of 30 ml of plasma. The plasma is divided into 5 tubes, each tube containing 6 ml of plasma. Samples are processed according to the following:
    [0134] - Control-activator sample: PRP that is activated with PRP supernatant (333 gl) and 20 gl of 10% calcium chloride for each 1 ml of PRP.
    [0135] - Sample formulation 1: The temperature of PRP is raised to 51 ° C.
    [0136] Subsequently, it is centrifuged at a speed of 580 g, for a time of 8 minutes and at room temperature. 2/3 of the initial volume is removed and the platelet and protein precipitate is resuspended in the remaining 1/3 of the initial volume. The formulation is activated with the PRP supernatant (333 gl) and 20 gl of 10% calcium chloride for each 1 ml of the PRP.
    [0137] - Sample formulation 2: The temperature of PRP is raised to 51 ° C.
    [0138] Subsequently, it is centrifuged at a speed of 580 g, for a time of 8 minutes and at room temperature. 1/2 of the initial volume is removed and the platelet and protein precipitate is resuspended in the remaining 1/2 of the initial volume. The formulation is activated with the following activator / formulation volume ratios:
    [0139] 1. PRP supernatant (333 gl) and 20 gl of 10% calcium chloride for each 1ml of PRP (formulation 2).
    [0140] 2. PRP supernatant (235.8 gl) and 14.2 gl of 10% calcium chloride for each 1ml of PRP (formulation 2 A)
    [0141] 3. PRP supernatant (166.7 gl) and 10 gl of 10% calcium chloride for each 1ml of PRP (formulation 2 B)
    [0143] - Tisseel® Sample: A commercial Tisseel® adhesive and sealant (Baxter S. L., Valencia, Spain) has been purchased and used according to the manufacturer's instructions.
    [0145] Two glass slides with the samples have been glued described above after activation. The samples have been incubated in distilled water and then the strength of the adhesion of the formulation has been measured using weights in grams. Figure 5 shows the adhesion strength of formulations according to the present invention can also be improved by optimizing the volume of activator added (Formulation 2 B). The results also show that the adhesion strength of the formulation according to the invention (Formulation 2 B) is comparable with the commercial sealant of the Tisseel® brand.
    [0147] Example 6
    [0149] The starting point is a sample of 7 tubes (9ml) that hermetically contain blood drawn from a patient. The tubes are centrifuged at a speed of 580 g, for a time of 8 minutes and at room temperature. As a consequence of centrifugation, the blood contained in each tube is divided into several fractions. The upper fraction, or platelet-rich plasma (PRP) fraction, is extracted into a blank tube, obtaining a total of 24 ml of plasma. The plasma is divided into 4 tubes, each tube containing 6 ml of plasma. Samples are processed according to the following:
    [0151] - Control-activator sample: PRP that is activated with PRP supernatant (333 µl) and 20 µl of 10% calcium chloride for each 1 ml of PRP.
    [0152] - Sample formulation 1: The temperature of PRP is raised to 51 ° C.
    [0153] Subsequently, it is centrifuged at a speed of 580 g, for a time of 8 minutes and at room temperature. 2/3 of the initial volume is removed and the platelet and protein precipitate is resuspended in the remaining 1/3 of the initial volume. The formulation is activated with the PRP supernatant (333 µl) and 20 µl of 10% calcium chloride for each 1 ml of PRP.
    [0154] - Sample formulation 2: The temperature of PRP is raised to 51 ° C.
    [0155] Subsequently, it is centrifuged at a speed of 580 g, for a time of 8 minutes and at room temperature. 1/2 of the initial volume is removed and the platelet and protein precipitate is resuspended in the remaining 1/2 of the initial volume. I know Activates the formulation with the following activator / formulation volume ratios:
    [0156] 1. PRP supernatant (333 gl) and 20 gl of 10% calcium chloride for each 1ml of PRP (formulation 2).
    [0157] 2. PRP supernatant (166.7 gl) and 10 gl of 10% calcium chloride for each 1ml of PRP (formulation 2 B)
    [0159] - Tisseel® Sample: A commercial Tisseel® adhesive and sealant (Baxter S. L., Valencia, Spain) has been purchased and used according to the manufacturer's instructions.
    [0161] Biological samples of pig skin have been prepared. The skin samples have been glued to a support using a universal adhesive. Then two skin specimens have been glued with the samples described above. The adhesion strength of the formulation was then measured using gram weights and hanging them on the support of a skin specimen. Figure 6 shows the novelty and effectiveness of the invention in improving adhesion strength and that adhesion capacity can be further increased by optimizing the volume of activator added (Formulation 2B). The results also indicate that the adhesion force of the formulation according to the present invention (Formulation 2 B) is comparable with the adhesion force of the commercial sealant of the Tisseel® brand.
    权利要求:
    Claims (12)
    [1]
    1. Method of preparing an adhesive formulation from an initial blood composition, characterized in that it comprises the steps of:
    a) obtaining an initial blood composition, of human or animal origin, containing platelets;
    b) raising the temperature of the initial blood composition to a temperature of 40 to 55 ° C; and
    c) activating platelets and forming a fibrin-containing formulation.
    [2]
    2. Method according to claim 1, characterized in that the initial blood composition is a blood plasma rich in platelets.
    [3]
    3. Method according to claim 1, characterized in that the initial blood composition is a blood plasma rich in released growth factors.
    [4]
    4. Method according to claim 1, characterized in that in step b) the temperature of the initial blood composition is raised to a temperature of 40 to 53 ° C.
    [5]
    5. Method according to claim 1, characterized in that it comprises an additional step of centrifuging the blood composition after step b).
    [6]
    6. Method according to claim 5, characterized in that it comprises an additional step of removing part of the volume of the initial composition after step b).
    [7]
    7. Method according to claim 1, characterized in that the step of activating platelets comprises adding at least one of: calcium chloride, thrombin, sodium gluconate, collagen, blood plasma supernatant, and factor-rich blood plasma supernatant of increase.
    [8]
    8. Method according to claim 1, characterized in that the formulation comprises one or more bioactive agents selected from proteins, peptides, nucleic acids, polysaccharides, lipids, non-protein organic substances and inorganic substances.
    [9]
    9. Method according to claim 1, characterized in that the formulation comprises one or more biodegradable polymers selected from: hyaluronic acid, hyaluronate salts, chondroitin 4 sulfate, chondroitin 6 sulfate, dextran, silica gel, alginate, hydroxypropylmethylcellulose, derivatives chitin, preferably chitosan, xanthan gum, agarose; polyethylene glycol (PEG), polyhydroxyethylene methacrylate (HEMA), synthetic or natural proteins, collagens.
    [10]
    10. Method according to claim 1, characterized in that the formulation comprises one or more organic polymers selected from the group of polycarpolactone, polyglycolic, polylactic, and their co-polymers.
    [11]
    11. Method according to claim 1, characterized in that the formulation comprises one or more of the following agents: antibiotics, antimicrobials, anticancer agents, analgesics, growth factors, hormones.
    [12]
    12. Method according to claim 1, characterized in that the formulation comprises one or more inorganic components selected from the group of calcium salts, magnesium salts, and / or strontium salts.
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    同族专利:
    公开号 | 公开日
    EP3925613A1|2021-12-22|
    CO2021010335A2|2021-08-19|
    AR118039A1|2021-09-15|
    TW202045191A|2020-12-16|
    ES2778798B2|2021-10-13|
    CA3129633A1|2020-08-20|
    WO2020165473A1|2020-08-20|
    US20200254138A1|2020-08-13|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    ES2221770A1|2002-04-19|2005-01-01|Eduardo Anitua Aldecoa|Preparation of a tissue regeneration agent comprises employing growth factors and protein rich coagulated materials|
    ES2369945B1|2011-07-29|2012-10-15|Eduardo Anitua Aldecoa|PROCEDURE FOR OBTAINING A COMPOSITION CONTAINING GROWTH FACTORS FROM A BLOOD COMPOUND, AND COMPOSITION OBTAINABLE BY SUCH PROCEDURE.|
    US20150037430A1|2013-08-01|2015-02-05|Biotechnology Institute, I Mas D, S.L.|Formulation of a blood composition that is rich in platelet and/or growth factors and contains gelled proteins, and a method for its preparation|
    AR022333A1|1999-01-26|2002-09-04|Anitua Aldecoa Eduardo|OSEO FABRIC REGENERATOR|
    ES2633815B1|2016-03-23|2018-07-06|Biotechnology Institute I Mas D, S.L.|FORMULATION OF TOPICAL APPLICATION, RICH IN PLATES AND / OR GROWTH FACTORS AND A METHOD OF PREPARATION OF THE SAME|
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    PCT/ES2020/070048| WO2020165473A1|2019-02-11|2020-01-22|Tissue formulation or adhesive obtained from a blood composition containing platelets, and method of preparing such formulation|
    EP20708156.3A| EP3925613A1|2019-02-11|2020-01-22|Tissue formulation or adhesive obtained from a blood composition containing platelets, and method of preparing such formulation|
    TW109103431A| TW202045191A|2019-02-11|2020-02-05|Tissular formulation or adhesive obtained from a blood composition containing platelets, and method for the preparation of said formulation|
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    US16/787,190| US20200254138A1|2019-02-11|2020-02-11|Tissular formulation or adhesive obtained from a blood composition containing platelets, andmethod for the preparation of said formulation|
    CONC2021/0010335A| CO2021010335A2|2019-02-11|2021-08-05|Formulation or tissue adhesive obtained from a blood composition containing platelets, and method of preparing said formulation|
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